| Gene information | Literature | Expression | lncRNA | Mutation | Homolog |
Basic Information | |
|---|---|
Gene ID | 406966 |
Name | MIR191 |
Synonymous | microRNA 191;MIR191;microRNA 191 |
Definition | hsa-mir-191 |
Position | 3p21.31 |
Gene type | ncRNA |
Title | Abstract |
| hsa-miR-191 is a candidate oncogene target for hepatocellular carcinoma therapy. | Hepatocellular carcinoma (HCC) is generally a fatal disease due to a paucity of effective treatment options. The identification of oncogenic microRNAs that exert pleiotropic effects in HCC cells may offer new therapeutic targets. In this study, we have identified the human microRNA miR-191 as a potential target for HCC therapy. Inhibition of miR-191 decreased cell proliferation and induced apoptosis in vitro and significantly reduced tumor masses in vivo in an orthotopic xenograft mouse model of HCC. Additionally, miR-191 was found to be upregulated by a dioxin, a known liver carcinogen, and was found to be a regulator of a variety of cancer-related pathways. Our findings offer a preclinical proof of concept for miR-191 targeting as a rational strategy to pursue for improving HCC treatment. |
| MicroRNA-191 targets N-deacetylase/N-sulfotransferase 1 and promotes cell growth in human gastric carcinoma cell line MGC803. | As a family of post-transcriptional regulator of gene expression, the microRNAs (miRNAs) control a wide array of biological processes including cell differentiation, proliferation and apoptosis, and the dysregulation of miRNAs is a hallmark of cancer. Here, we found that the microRNA-191 (miR-191) was at a high-expression level in human gastric adenocarcinoma cell line MGC803 and human gastric cancer tissues. The results of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and colony formation assays showed that miR-191 could promote cell growth and suppress apoptosis of MGC803 cells. The N-deacetylase/N-sulfotransferase 1 (NDST1) was confirmed to be a direct target gene of miR-191 by enhanced green fluorescent protein reporter experiment. The mRNA and protein levels of NDST1 were inversely correlated with miR-191 in MGC803 cells, suggesting the negative regulation of NDST1 by miR-191. Furthermore, NDST1 played an inhibitory role and could suppress MGC803 cell proliferation. Our findings suggested that miR-191 could act as an oncogene in MGC803 cells, and the cellular function was partially due to its negative regulation of NDST1. |