| Gene information | Literature | Expression | lncRNA | Mutation | Homolog |
Basic Information | |
|---|---|
Gene ID | 10801 |
Name | SEPT9 |
Synonymous | septin 9;SEPT9;septin 9 |
Definition | MLL septin-like fusion protein MSF-A|Ov/Br septin|ovarian/breast septin|septin D1|septin-9 |
Position | 17q25 |
Gene type | protein-coding |
Title | Abstract |
| Sint1, a common integration site in SL3-3-induced T-cell lymphomas, harbors a putative proto-oncogene with homology to the septin gene family. | The murine retrovirus SL3-3 is a potent inducer of T-cell lymphomas when inoculated into susceptible newborn mice. Previously, DNAs from twenty SL3-3-induced tumors were screened by PCR for provirus integration sites. Two out of 20 tumors demonstrated clonal provirus insertion into a common region. This region has now been isolated and characterized. The region, named SL3-3 integration site 1 (Sint1), maps to the distal end of mouse chromosome 11, corresponding to human chromosome 17q25, and may be identical to a mouse mammary tumor virus integration site in a T-cell lymphoma, Pad3. Two overlapping genomic lambda clones spanning about 35 kb were isolated and used as a starting point for a search for genes in the neighborhood of the virus integration sites. A genomic fragment was used as a hybridization probe to isolate a 3-kb cDNA clone, the expression of which was upregulated in one of two tumors harboring a provirus in Sint1. The cDNA clone is predicted to encode a protein which shows 97.0% identity to a human septin-like protein encoded by a gene which has been found as a fusion partner gene of MLL in an acute myeloid leukemia with a t(11;17)(q23;q25). Together these findings raise the possibility that a proto-oncogene belonging to the septin family, and located about 15 kb upstream of the provirus integration sites, is involved in murine leukemia virus-induced T-cell lymphomagenesis. |
| Alternative splicing, expression, and gene structure of the septin-like putative proto-oncogene Sint1. | Sint1 (sept9), a murine gene of the septin family, was previously isolated as a putative proto-oncogene involved in T-cell lymphomagenesis. We now present its genomic structure and report on nine exons shared by ALL identified variants and at least four alternatively spliced 5 exons. Northern blot analyses using a Sint1 cDNA probe showed in almost ALL examined tissues two predominant transcripts of 3 and 4 kb. Exon-specific expression analyses assigned one of the 5 exons to the 4 kb transcript, while the other 5 exons seem to represent novel, tissue-specific, weakly expressed transcripts of different sizes, and none of them appear to hybridize to the major 3 kb transcript. Whole-mount in situ hybridization on post-implantation embryos revealed several areas strongly expressing Sint1, including neural crest cells, cephalic mesenchyme, and mesenchymal cells in the developing limb. A clustering of proviruses in four independent retrovirally induced tumors point to a region of about 3 kb around the most upstream exon as important for proviral deregulation of Sint1. |