| General information | Literature | Expression | lncRNA |Regulation | Mutation | Homolog | Interaction |
Basic Information | |
|---|---|
Gene ID | 5948 |
Name | RBP2 |
Sentence | From PubMed database |
| Retinoblastoma-binding protein 2 induces epithelial-mesenchymal transition in esophageal squamous cancer cells. | OBJECTIVES: To investigate the effect of retinoblastoma-binding protein 2 (RBP2) on epithelial-mesenchymal transition (EMT) in esophageal squamous cancer cells and to compare the effect of RBP2 in lung squamous cancer cells and esophageal squamous cancer cells. RESULTS: When transfected with RBP2 siRNA, the migrated cells were 36.3 +/- 6.03 by transwell migration assay, compared to 107 +/- 6.7 cells in the control group. The mRNA level of epithelial cadherin (E-cadherin) was 1.54 +/- 0.14 times higher than in the control group, and that of neural cadherin (N-cadherin) fell to 0.76 +/- 0.03 times. The relative luciferase activity of E-cadherin promoter rose to 3.84 +/- 0.23 times. Correspondingly, the expression of E-cadherin protein increased and that of N-cadherin protein decreased. When SK-MES-1 cells were transfected with RBP2 siRNA, their relative mRNA level of E-cadherin was 8.6 +/- 0.37 times as high as that in control group, which was higher than that in Eca-109 cells. The E-cadherin protein was also greater in SK-MES-1 cells. CONCLUSION: RBP2 could induce EMT in esophageal cancer cells and exert a greater effect on the expression of E-cadherin in lung squamous cells than in esophageal squamous cells. |