| General information | Literature | Expression | lncRNA |Regulation | Mutation | Homolog | Interaction |
Basic Information | |
|---|---|
Gene ID | 406941 |
Name | MIR149 |
Sentence | From PubMed database |
| miR-149 promotes epithelial-mesenchymal transition and invasion in nasopharyngeal carcinoma cells. | OBJECTIVE: To investigate the function and mechanism of miR-149 in nasopharyngeal carcinoma (NPC). METHODS: The expression of miR-149 was examined by real-time PCR and calculated by 2(-triangle uptriangle upCt) method. The cell proliferation was analyzed by MTT assay. The cell migration and invasion were shown by the wound healing assay and transwell migration assay, and the expression of E-cadherin was detected by Western blot. RESULTS: The expression of miR-149 was higher in NPC cell lines 5-8F and 6-10B than that in normal immortalized nasopharyngeal epithelial NP69. MTT assay showed that miR-149 promoted the proliferation of NPC cell lines. The wound healing assay showed miR-149 promoted the mobility and invasion of NPC cell lines. Inhibition of miR-149 reduced the ability of NPC cell lines to proliferate and invade. miR-149 downregulated the expression of E-cadherin, whereas antagomir which mediated knockdown of miR-149 significantly upregulated the expression of E-cadherin. CONCLUSION: miR-149 might be involved in the invasion and metastasis of NPC through regulation of epithelial-mesenchymal transition (EMT). |