| General information | Literature | Expression | Regulation | Mutation | Homolog | Interaction |
Basic Information | |
|---|---|
Gene ID | 407021 |
Name | MIR29A |
Synonymous | microRNA 29a;MIR29A;microRNA 29a |
Definition | microRNA 29 |
Position | 7q32.3 |
Gene type | ncRNA |
Title | Abstract |
| miR-29 and miR-30 regulate B-Myb expression during cellular senescence. | cellular senescence is a form of irreversible growth arrest and a major tumor suppressor mechanism. We show here that the miR-29 and miR-30 microRNA families are up-regulated during induced and replicative senescence and that up-regulation requires activation of the Rb pathway. expression of a reporter construct containing the 3UTR of the B-Myb oncogene is repressed during senescence, and repression is blocked by mutations in conserved miR-29 and miR-30 binding sites in the B-Myb 3UTR. In proliferating cells, transfection of miR-29 and miR-30 represses a reporter construct containing the wild-type but not the mutant B-Myb 3UTR, and repression of the mutant 3UTR is reinstituted by compensatory mutations in miR-29 and miR-30 that restore binding to the mutant sites. miR-29 and miR-30 introduction also represses expression of endogenous B-Myb and inhibits cellular DNA synthesis. Finally, interference with miR-29 and miR-30 expression inhibits senescence. These findings demonstrate that miR-29 and miR-30 regulate B-Myb expression by binding to its 3UTR and suggest that these microRNAs play an important role in Rb-driven cellular senescence. |