84666

RETNLB

resistin like beta;RETNLB;resistin like beta

C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 2|colon and small intestine-specific cysteine-rich protein|colon carcinoma-related gene protein|cysteine-rich secreted A12-alpha-like protein 1|cysteine-rich secreted protei

3q13.1

protein-coding

AIM: To investigate the effects of resistin-like molecule beta (RELMbeta) over-expression on the invasion, metastasis and angiogenesis of gastric cancer cells. METHODS: Human RELMbeta encoding expression vector was constructed and transfected into the RELMbeta lowly-expressed gastric cancer cell lines SGC-7901 and MKN-45. Gene expression was measured by Western blotting, reverse transcription polymerase chain reaction (PCR) and real-time quantitative PCR. Cell proliferation was measured by 2-(4,5-dimethyltriazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetry, colony formation and 5-ethynyl-20-deoxyuridine incorporation assays. The in vitro migration, invasion and metastasis of cancer cells were measured by cell adhesion assay, scratch assay and matrigel invasion assay. The angiogenic capabilities of cancer cells were measured by tube formation of endothelial cells. RESULTS: Transfection of RELMbeta vector into SGC-7901 and MKN-45 cells resulted in over-expression of RELMbeta, which did not influence the cellular proliferation. However, over-expression of RELMbeta suppressed the in vitro adhesion, invasion and metastasis of cancer cells, accompanied by decreased expression of matrix metalloproteinase-2 (MMP-2) and MMP-9. Moreover, transfection of RELMbeta attenuated the expression of vascular endothelial growth factor and in vitro angiogenic capabilities of cancer cells. CONCLUSION: Over-expression of RELMbeta abolishes the invasion, metastasis and angiogenesis of gastric cancer cells in vitro, suggesting its potentials as a novel therapeutic target for gastric cancer.