| General information | Literature | Expression | Regulation | Mutation | Homolog | Interaction |
Basic Information | |
|---|---|
Gene ID | 3911 |
Name | LAMA5 |
Synonymous | laminin, alpha 5;LAMA5;laminin, alpha 5 |
Definition | laminin alpha-5 chain|laminin subunit alpha-5|laminin-10 subunit alpha|laminin-11 subunit alpha|laminin-15 subunit alpha |
Position | 20q13.2-q13.3 |
Gene type | protein-coding |
Title | Abstract |
| melanoma | Melanoma cells express and interact with laminins (LMs) and other basement membrane components during invasion and metastasis. In the present study we have investigated the production and migration-promoting activity of laminin isoforms in melanoma. Immunohistochemistry of melanoma specimens and immunoprecipitation/western blotting of melanoma cell lines indicated expression of laminin-111/121, laminin-211, laminin-411/421, and laminin-511/521. Laminin-332 was not detected. In functional assays, laminin-111, laminin-332, and laminin-511, but not laminin-211 and laminin-411, strongly promoted haptotactic cell migration either constitutively or following stimulation with insulin-like growth factors. Both placenta and recombinant laminin-511 preparations were highly active, and the isolated recombinant IVa domain of LMalpha5 also promoted cell migration. Function-blocking antibodies in cell migration assays revealed alpha6beta1 integrin as the major receptor for laminin-111, and both alpha3beta1 and alpha6beta1 integrins for laminin-332 and laminin-511. In contrast, isolated LMalpha5 IVa domain-promoted melanoma cell migration was largely mediated via alphaVbeta3 integrin and inhibited by RGD peptides. Given the ubiquitous expression of alpha5 laminins in melanoma cells and in melanoma-target tissues/anatomical structures, as well as the strong migration-promoting activity of these laminin isoforms, the alpha5 laminins emerge as putative primary extracellular matrix mediators of melanoma invasion and metastasis via alpha3beta1 and other integrin receptors. |