1460

CSNK2B

casein kinase 2, beta polypeptide;CSNK2B;casein kinase 2, beta polypeptide

CK II beta|CSNK2B-LY6G5B-532|CSNK2B-LY6G5B-560|Casein kinase II beta subunit|alternative name: G5a, phosvitin|casein kinase II b subunit splicing isoform 318|casein kinase II b subunit splicing isoform 660|casein kinase II b subunit splicing isoform 806|c

6p21.3

protein-coding

OBJECTIVE: To investigate the expression of casein kinase 2beta (ck2beta) in colorectal cancer in relation to the metastatic ability of the cancer cells. METHODS: The expression of ck2beta in 46 normal colorectal mucosa, 20 colorectal adenomas and 66 colorectal cancers were detected immunohistochemically. In colorectal cancer cells, Ck2beta protein expression was knockdown by RNA interference using ck2beta-specific small interfering RNA (siRNA) and the interference efficiency was assessed by Western blotting. The effect of ck2beta gene knockdown on the proliferation of the colorectal cancer cells was tested with colony formation assay, and the changes in the invasive ability of the cells were observed using Transwell chamber assay. RESULTS: Negative or weak ck2beta expression was detected in normal colorectal mucosa, with nuclear positivity in 8.7% and cytoplasmic positivity in 13.0% of the cases. Colorectal adenomas showed moderate ck2beta expression, with 60% cases showing positivity in the cell nuclei and 40% in the cytoplasm. In colorectal cancers, significantly stronger expression of ck2beta was found than that in colorectal adenomas and normal colorectal mucosa (P<0.05), and 75.8% cases showed positivity in the cell nuclei and 62.1% showed cytoplasmic positivity; the expression of ck2beta protein in colorectal cancers with lymph node metastasis was even higher (P<0.05). Ck2beta knockdown obviously inhibited the proliferation and invasiveness of colorectal cancer cells in vitro. CONCLUSION: The high expression of ck2beta in colorectal cancer is closely correlated to the carcinogenesis and metastasis of the tumor.